The goal of this project is to develop compact mathematical model to describe the most important aspects of glucose, amino acids and oxygen metabolism in hiPSc 3D expansion process by taking into account stem cell aggregation phenomenon and heterogeneity of the culture. Based on the developed mathematical model, some optimization procedures will be carried out to identify the rational culture parameters for the cell expansion process, such as feeding modes, oxygen tension and cell aggregation control. The improved culture cultivation parameters should allow achieving higher concentration of undifferentiated (hiPSc) cells at the end of cultivation without significant differentiation of stem cells. Project partner: Laboratory of Bioprocess Systems Engineering, Osaka University (Japan).
Project coordinator: Kaunas University of Technology
Project partners: Osaka University